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The treated PTFE films were evaluated by dynamic water contact angle measurement, scanning electron microscopy, X-photoelectron microscopy and cellular adhesion.
After NPs preparation by emulsification diffusion, their size, encapsulation efficiency (EE%), drug release profile, morphology, and cytotoxicity are characterized by dynamic light scattering, spectrophotometry, transmission electron microscopy, and cellular viability assay/transepithelial electrical resistance measurement, respectively.
The authors thank Philippe Hulin and the "confocal microscopy and cellular imaging platform" of IFR26 for time lapse study.
These observations are consistent with previous studies that localized TORC1 via immunogold electron microscopy and cellular fractionation (Chen and Kaiser 2003; Reinke et al. 2004).
Following mass measurement, cells were fixed (4% paraformaldehyde in PBS, 30 min) for volume estimation with confocal microscopy and cellular identification through immunocytochemistry.
Retrieved synovial tissue morphology was graded using light microscopy and cellular composition was assessed using immunohistochemistry. Length of implantation was shorter in the DMN group versus MoM THA group (21.3 [8.4] months versus 43.6 [13.8] months respectively; p < 0.005) suggesting differences in implant performance.
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We showed that there was considerable atrophy in the muscles of death grip ants, using both light microscopy and sub-cellular transmission microscopy.
After the first screen with fluorescence microscopy, the precise localization and cellular composition of the clusters was determined.
The best data sets are provided by combining isotope tracing demonstrating uptake in an organ and microscopy imaging cellular distribution.
However, FITC labeling of the SiO2 nanoparticles and subsequent analysis using flow cytometry and fluorescence microscopy revealed cellular uptake of the particles both in the presence and in the absence of serum.
The synergy between X-ray crystallography and cellular fluorescence microscopy has become particularly important, precisely because the results from these two methods diverged and highlighted the complexity of the challenge.
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