The sections were evaluated by light microscopic examination, and cellular localisation and intensity (negative=0, weak=1, moderate=2, strong=3) of immunostaining in each section were assessed by two observers (RD and GIM).
In recent years, the deluge of complicated molecular and cellular microscopic images creates compelling challenges for the image computing community.
Despite all this, the time of recovery is age dependent, presumably reflecting subclinical (even microscopic) tissue, cellular and subcellular damage (Howlett and Rockwood 2013).
Similar to Tsc2c/− /hGFAP2- Cre+ mice (Way et al., 2009), Tsc1c/− /hGFAP2- Cre+ P15 mutant brain showed clear signs of megalencephaly, lamination defects and microscopic cellular alterations (n=5 mice; Fig. 2A).
Hence, at both the macro- and microscopic levels, cellular MRI appears to be a relatively reliable tool to monitor the migration of engrafted cells (Politi 2007; Lee et al. 2004; Mligiliche et al. 2005; Zhang et al. 2003, 2004).
Utilizing epi-third-harmonic-generation (THG) and epi-second-harmonic-generation (SHG) signals, the microscopic cellular and subcellular structures of epithelium and lamina propria in human oral mucosa can be obtained without fluorescence signals, with a lateral spatial resolution better than 500 nm even at 280 μm beneath the surface.
This provides an important bridge between observations at microscopic (cellular) and macroscopic (whole-brain) scales.
What are the molecular and cellular driving forces supporting the microscopic structural differences between neuropathic and ischemic ulcers beds?
For mutational profiling, DNA was extracted from cells microdissected from cytology slides guided by microscopic feature (cellular atypia when present) and from a portion of each cell-free supernatant sample that is normally discarded during preparation of cytology specimens.
Total scores of intensity of protein expression and cellular positivity were determined from different microscopic fields.
