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To facilitate image analysis researchers used fluorescence microscopy and cells expressing GFP from a plasmid under the control of the pykF promoter, allowing precise software-based measurement of both the long and short axis of the cells.
Incubation of the cells was stopped after 24 h, cell growth was examined with light microscopy and cells were fixed with 4% paraformaldehyde (PFA, Sigma-Aldrich) for 20 minutes at room temperature.
48-hpf intact hearts were imaged by confocal microscopy and cells counted for all planes sampled.
The expression of G3BP GFP was analysed by fluorescence microscopy and cells were scored for stress granule formation.
Cell divisions were recorded with time-lapse microscopy, and cells were subsequently fixed and stained for the differentiation markers GFAP and Map2.
Cells on coverslips were fixed for fluorescence microscopy and cells attached to the bottom of six-well plates for transmission electron microscopy analyses.
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Cell attachment and growth were evaluated by scanning electron microscopy and cell viability assays, respectively.
Cytoskeletal phenotypes were assessed with immunofluorescent microscopy and cell attachment assays.
Both methods involve serial histological sectioning, triple label immunohistochemistry, laser confocal microscopy and cell counting with the optical disector/fractionator.
The behavior of DPSCs cultured on the scaffold was evaluated by scanning electron microscopy and cell differentiation, by histological analysis.
Morphology of the foams was also observed using scanning electron microscopy and cell size distributions of the materials were determined.
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