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Passing through the microlens, the transmitted speckle pattern is collected by a microscope objective and measured by a CCD.
The PL was collected using the same microscope objective and separated by a dichroic mirror from the excitation path.
The emitted light from fluorescent tracer particles is then collected by the microscope objective and recorded by a camera.
Normal Raman experiments were performed using a Bruker Senterra Raman spectrometer equipped with an Olympus 100x long working distance microscope objective and a charge-coupled device (CCD) detector.
Raman measurements were performed using a Bruker Senterra Raman spectrometer coupled with an LMPlanFL Olympus 50× long working distance microscope objective and a charge-coupled device (CCD) detector.
The spatial resolution is 2 μm when using the 638 nm laser with a 50× microscope objective and the maximum laser power used was always less than 0.1 mW at the sample.
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Fine, thin and flat magnetic poles allow the use of high magnification microscope objectives, and flexible software to control the direction and pattern of applied forces supports a variety of experimental situations.
By using high NA microscope objectives and laser pulse energies close to the ablation threshold, the lateral extend of the modified material is limited to less than 1 µm[ 3, 7].
This setup is based on a modified Mach-Zehnder interferometer and it includes a 632.8nm, 0.5 mW Helium-Neon laser, two retro-reflectors inserted in the beam paths, two 40 ×, 0.65 numerical-aperture microscope objectives, and a monochromatic CMOS camera with 5.2µm square pixels (Thorlabs DCC1545M).
Future improvements of cell tracking techniques in vivo requires, among others, the development of specifically designed new fluorophores with improved two-photon absorption cross-sections and the optimization of microscope objectives and detector light paths to maximize the collection of emitted fluorescence photons.
The stage is customized to fit under a microscope objective lens and between the TX and RX antennas of a custom-built portable RF device.
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