Sentence examples for lens objective and from inspiring English sources
For time lapse imaging, we used a Zeiss Axiovert 200 M microscope equipped with a ×20 lens objective and an Axiocam camera (Additional file 2) or a Leica SPE confocal microscope using a ×40 lens objective (Additional file 3).
The stained sections were visualized with a Nikon Eclipse TE2000-U fluorescence microscope (Nikon, Melville, NY, USA) using a 40× lens objective, and the captured fluorescence images were analyzed using MetaMorph software (Molecular Devices, Sunnyvale, CA, USA).
Using an Ultra VIEW VoX spinning disc confocal microscope (PerkinElmer, Waltham, MA, USA), hippocampal PSD-95 immunoreactive puncta were imaged with a 60× lens objective and digitally magnified to × 100.
Invasive cells were collected for 4 hours, then the contents of the needles were extruded onto a coverslip by using 0.5 μg/ml 4',6-diamidino-2-phenylindole in PBS to quantify the number of invasive cells with an Olympus IX70 inverted microscope with a 10× lens objective and a 0.30 numerical aperture (NA) (Olympus America, Melville, NY, USA).
The optical components such as scan mirrors, scan lenses, objective and GRIN lens system are compatible with this new excitation source.
The single lens collimator and double lens objective comprise an infinite conjugate optical imaging configuration [ 33].
The fluorescence signal from the sample is epi-collected through the GRIN lenses and the objective, and is reflected by a dichroic beam splitter (FF705-Di01, Semrock Inc).
A scan lens and objective lens focus the raster scanning beam onto the tissue.
In order to scan a 13.2 x 13.2 mm field of view, the distance between the tube lens and objective must be reduced to less than 500 mm.
The fluorescence signal from the sample is epi-collected through the GRIN lenses and the microscope objective, and is reflected by a dichroic beam splitter (FF705-Di01, Semrock Inc).
An additional convergent lens is provided between the objective and the eyepiece to right the image.
