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Exact(13)
The 106 CFU/mL microbial suspension was then added to each tube containing the glass slide pieces.
The turbidity of actively growing microbial suspension was adjusted to match the turbidity standard of 0.5 Mc Farland units (Kaur and Arora 2009).
Briefly, 75 µL of an agar/mucin solution [5% porcine mucin type III (Sigma-aldrich, Diegem, Belgium), 0.8% agar (BD, Erembodegem, Belgium)] was brought on the porous membrane (0.4 µm) of a 24-well plate Transwell® system (Corning Inc., NY, USA) and allowed to solidify for at least 30 min after which 20 µL of a microbial suspension was spotted on top of this agar/mucin layer (apical compartment).
The microbial suspension was mixed with peptides.
A 100 μL (10 cfu) of diluted microbial suspension was swabbed on nutrient agar plates.
Then 10 μL of the microbial suspension was transferred into the canal lumen using sterile needles and incubated at 37°C for 72 hours.
Similar(47)
The use of the test microbial suspension is to monitor the disinfection procedure and its performance is dependent on both the initial test microbial suspension population (N0) and the D-value [ 9, 10].
After PMA treatment, the genomic DNA of treated microbial suspensions was extracted and purified using the MasterPure DNA purification kit (Epicenter Technologies).
The test microbial suspensions were spread on Petri dishes and freshly prepared Ag and AuNPs samples were introduced.
Suitable concentrations of microbial suspensions were prepared from (1 for bacteria to 3 for yeast and fungi -day-old liquid stock cultures in cubated on a rotary shaker (100 rpm).
Aliquots of 10 µl of the microbial suspensions were applied to microscope slides, and binding of FITC-labeled Vg to the microbial cells was observed under an Olympus BX51 fluorescence microscope.
Related(20)
bacterial suspension was
algal suspension was
extract suspension was
challenge suspension was
microbial fermentation was
microbial autofluorescence was
microbial analysis was
microbial documentation was
microbial efficiency was
microbial life was
microbial injury was
microbial cosmos was
microbial density was
microbial activity was
microbial community was
microbial suspension sieved
microbial growth was
microbial colonization was
microbial consortium was
microbial ecology was
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