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The microbial analysis was conducted by the dilution-plate method (Shen et al. 1999).
Microbial analysis was started after observable biofilm in the gravel and root (i.e., ~ 3 days after setup).
Microbial analysis was carried out on vitreous samples in 5/6 eyes (83.3%) and on a corneal biopsy in 1 eye with the iris root abscess.
Microbial analysis was performed for 9 periodontal pathogens (Aggregatibacter actinomycetemcomitans (Aa), Porphyromonas gingivalis (Pg), Treponema denticola (Td), Tannerella forsythia (Tf), Prevotella intermedia (Pi), Peptostreptococcus micros (Pm), Fusobacterium nucleatum (Fn), Eubacterium nodatum (En), and Capnocytophaga gingivalis (Cg)).
Accordingly, we conducted a study ancillary to the Macrolide trial in which sputum collected from participants for microbial analysis was analysed for PGP and the ability to generate PGP from collagen.
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The mean, standard error of the mean and range for microbial analysis are presented in Table 4.
Data for the microbial analysis were log transformed, while N-mineralised and N- and P-losses were transformed into percentages before ANOVA.
The soil samples for microbial analysis were processed within two weeks of collection.
Getting the appropriate sample for microbial analysis is critical.
The data for differences between the digestion phase and the results of microbial analysis were analyzed using one-way analysis of variance (ANOVA) and presented as mean ± standard deviations.
Two series of soil samples for microbial analysis were taken immediately after the AC amendment of the soil, after 6 months, and after 3 years, and frozen at −20 °C with and without the addition of absolute ethanol 1 1 (v/v).
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