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More recently, the design of a handheld system for detecting microbial autofluorescence was described [14].
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Chlorophyll autofluorescence was detected with a 570-nm filter.
No autofluorescence was observed in untreated control cells.
By means of spectral unmixing, tissue autofluorescence was removed from the images.
The autofluorescence was proportional to the number of cells on the membrane and that there was no "shadowing" effect from piling of microbial cells (data not shown).
Autofluorescence was not subtracted.
At 514nm excitation, cell autofluorescence was insignificant.
No autofluorescence was detected in control roots.
Without staining, no significant autofluorescence was observed.
With these settings no autofluorescence was detected.
Finally, autofluorescence was quenched by Sudan Black B (Merck).
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