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Microbial lipases are used in the dairy industry extensively for the hydrolysis of milk fat, and current applications include acceleration of cheese ripening and lipolysis of butter, fat and cream (Aravindan et al. 2007).
Lipases are of plant, animal, and microbial origin, but microbial lipases are produced at industrial level and represent the most widely used class of enzymes in biotechnological applications and organic chemistry.
Most industrial microbial lipases are derived from fungi, especially the Aspergillus genus [ 10].
Microbial lipases are largely diversified in their enzymatic properties and substrate specificity, which make them potential source for industrial applications.
Moreover, microbial lipases are the most appealing ones mainly because of their applied properties, such as versatility and ease of mass production [ 5, 6].
Among different lipases, microbial lipases are preferable due to their broad substrate specificity, and higher stability with lower production costs compared to the lipases from plants and animals.
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The potential for commercial exploitation of a microbial lipase is determined by its yield, activity, stability and other characteristics.
In dairy industry, microbial lipases have been used in selective hydrolysis of fat triglycerides to release free fatty acids, which are used to develop flavored products, such as cheese, butter, margarine, milk chocolate, and sweets [ 3].
In view of the excellent catalytic activities and the huge application potential, more than 20 microbial lipases have been realized in large-scale commercial production.
Many microbial lipases have been identified as potential enzyme catalysts for biodiesel synthesis with a wide range of stabilities and catalytic efficiency [ 12].
Among different microbial enzymes, lipases are widely documented among bacteria, fungi, plants, and animals [ 17, 18].
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