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With the development of metabolic engineering, employment of a selected microbial host for accommodation of a designed biosynthetic pathway to produce a target compound has achieved tremendous success in the past several decades.
When these pathways are reconstituted in a heterologous microbial host for metabolic engineering purposes, the acyl-CoAs may be subject to undesirable hydrolysis by the host's native thioesterases, resulting in a waste of cellular energy and decreased intermediate availability, thus impairing bioconversion efficiency.
Although triterpene synthases have been expressed in microbial hosts such as S. cerevisiae there has been little effort made so far to engineer the metabolism of a microbial host for enhanced production of triterpenes.
This high yield makes it a promising microbial host for terpenoid biosynthesis (Julsing et al. 2007; Wagner et al. 2000).
Therefore, research into the progress of genetic engineering of MEP pathway enzymes in B. subtilis can provide more direct support for utilizing B. subtilis as a microbial host for terpenoid biosynthesis.
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Isoprenoids are used in many commercial applications and much work has gone into engineering microbial hosts for their production.
Comamonas species are attractive microbial hosts for bioremediation engineering due to their versatile metabolic capacity and diverse niches inhabited.
As alternative microbial hosts for butanol production with organic-solvent tolerant trait are in high demands, a butanol-tolerant bacterium, Bacillus subtilis GRSW2-B1, was thus isolated.
Subcellular organization, the sequestration of enzymes and metabolites within a synthetic organelle, is an important emerging technology for the optimization of microbial hosts for industrial applications.
Suitable microbial hosts for biofuel production must tolerate process stresses such as end-product toxicity and tolerance to fermentation inhibitors in order to achieve high yields and titers.
Engineering microbial hosts for the production of higher alcohols looks to combine the benefits of renewable biological production with the useful chemical properties of larger alcohols.
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