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After stringent washing, microarray slide is then subjected to fluorescence scanning.
The common theme is that the glass surface of the microarray slide is coated with active chemical groups, which facilitate the binding of end-modified DNA, typically amino modified.
The fluorescent intensities detected when the microarray slide is scanned is proportional to the concentration of these double stranded species.
Briefly, the hybridisation chamber is disassembled and the microarray slide is washed for 1 minute in GE wash buffer 1 at room temperature and then transferred to GE wash buffer 2 at 37°C for an additional 1 minute.
In addition, each microarray slide is composed of two arrays that hold two samples each (two-color arrays), allowing for the necessary inclusion of biological replicates in the experimental design.
The microarray slide is then used to detected expression level of mRNA related to the DNA printed on its surface by incubatiing of the microarray with a solution containing cDNA or RNA obtained from biological samples.
Similar(53)
Thereafter, the microarray slide was scanned using Agilent Microarray Scanner G2505B.
The microarray slide was placed probe side towards the target.
Then, one microarray slide was placed onto the gasket slide, with the active side facing down.
Replicate spots per microarray slide were summarised by calculating the arithmetic mean (Microsoft Excel 2003).
The rSEPs on the microarray slide were probed using patient sera according to previous descriptions [ 9].
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