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Methods of sample preparation and microarray hybridization have been described previously [21].
The LCM, RNA isolation, and microarray hybridization have been described in detail in previous work [ 14, 15].
Finally, we found that 12% of the genes recovered from our microarray hybridization have been described as genes differentially expressed between gastrula and syncytial blastoderm stages [ 26].
Recently the reproducibility, sensitivity and accuracy of a selection of different labeling methods in cDNA microarray hybridization have been compared [ 13- 16].
Studies based on cDNA library analysis and microarray hybridization have shown that only a subset of the mouse OR gene repertoire is expressed at detectable levels in the olfactory epithelium [ 9, 27].
Similar(55)
RNA used for microarray hybridization had an RNA Integrity Number of >8.5.
Microarray hybridization has been described in detail elsewhere [ 60].
Genotyping by microarray hybridization has proven to be challenging in species with complex genomes.
RNA used for microarray hybridization had an average RNA Integrity Number of 8.2.
First, though it has been pointed out that the probes undergo folding (Binder et al., 2004), the contribution of this to microarray hybridization has not been estimated quantitatively.
Microarray hybridization has been used to study the global gene expression from many different kinds of tissues and cell lines [ 1- 4].
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