Sentence examples for microarray hybridization given from inspiring English sources

DGE is very similar in the sampling approach to long oligonucleotide probe microarray hybridization, given that both techniques take short nucleic acid target sequences to sample expression of longer RNA molecules containing them, and both are 3' biased because they rely on extension of cDNAs from the polyA tail with a oligo-dT primer.

Indeed, of the total number probes on the microarrays only 2.73 – 4.97% from each mutant versus control hybridization gave log2 ratios above or below +/−0.4, suggesting the probes on the microarray had a low level of signal-to-noise.

Calibrating the appropriate signal and noise intensity thresholds for a given microarray hybridization requires the analysis of a set of positive and negative reference genes.

One microarray hybridization per animal was done giving six biological replicates per experimental point.

In previous studies, we used cross-species microarray hybridization to hybridize S. macrospora targets on N. crassa cDNA or oligonucleotide microarrays [ 8- 11]; however, in these analyses, less than 50% of all genes on the arrays gave a significant signal.

All the S. pneumoniae study isolates were hybridized once against the TIGR4 reference strain and the microarray hybridization experiments were repeated for isolates which gave poor hybridization results.

The laser microdissected cells were lysed and the RNA was isolated, amplified and biotinylated giving good quality aRNA for microarray hybridization.

For each microarray hybridization, amplified and dye-labeled RNA samples from a given time-point was co-hybridized with dye-labeled RNA from a common reference sample.

Potential target genes regulated by a given miRNA were identified by in silico prediction and microarray hybridization of Ago2 coimmunoprecipitates.

c The relative transcriptional differences between fungicide-treatment vs. untreated control given as log2 transformed fold-change as determined by microarray hybridization and by qRT-PCR.

Albert, T.J. et al. Direct selection of human genomic loci by microarray hybridization.