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For cDNA synthesis and microarray hybridization, refer to Whitfield et al. [5].
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The combined data reflect 5692 microarray hybridization measurements (referred to as experiments) in humans, 334 in flies, 1209 in worms, 753 in yeast, 649 in plants, and 293 in bacteria.
Albert, T.J. et al. Direct selection of human genomic loci by microarray hybridization.
Labelled DNA was then genotyped using DNA microarray hybridization.
Vontas, J. et al. Transcriptional analysis of insecticide resistance in Anopheles stephensi using cross-species microarray hybridization.
Reimer, J. J. & Turck, F. Genome-wide mapping of protein-DNA interaction by chromatin immunoprecipitation and DNA microarray hybridization (ChIP-chip).
Microarray hybridization was performed in the Roswell Park Genomics Shared Resource using the Illumina human Bead-ChIP array according to the manufacturer's instructions.
Microarray hybridization was performed at 65°C for 17 h in Agilent's SureHyb hybridization chambers.
About 2 μg RNA was isolated from anthers and used for RNA labeling and microarray hybridization.
RNA isolation, cDNA synthesis, and microarray hybridization were performed according to previous protocols [45].
Microarray hybridization, washing, and staining were conducted following the standard procedure described in the GeneChip® Expression Analysis Technical Manual (Affymetrix).
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