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Allele specific amplification in a homogeneous format or combined to microarray hybridization are powerful approaches for SNP genotyping.
Hooyberghs et al. [35] showed that the nearest-neighbor parameters of solution hybridization and microarray hybridization are well correlated (r = 0.839) for probes of 30 oligonucleotides in length.
GeneChip array design and detailed protocols for microarray hybridization are available from Affymetrix.
Details of probe design and microarray hybridization are available in the Supporting Information.
In the last years numerous studies on the physical background of microarray hybridization are published with the perspective of developing improved analysis algorithms [ 6- 12].
Correlations of log2 fold-change values in response to UV and AgNO3 treatment obtained by microarray hybridization are shown in Figure 2.
Similar(51)
Microarray hybridization was performed in the Roswell Park Genomics Shared Resource using the Illumina human Bead-ChIP array according to the manufacturer's instructions.
Microarray hybridization was performed at 65°C for 17 h in Agilent's SureHyb hybridization chambers.
RNA isolation, cDNA synthesis, and microarray hybridization were performed according to previous protocols [45].
All samples for microarray hybridization were processed at the Roche NimbleGen Service Laboratory.
Microarray hybridization was carried out in an ArrayBooster Hybridization Station (Advalytix, Brunnthal, Germany).
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