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To validate our miRNA microarray findings, we performed real-time PCR on 6 miRNAs of interest.
To validate microarray findings, we selected a few dozens of genes within different clusters for quantitative RT-PCR (qRT-PCR) analysis on an independent set of RNA samples.
To independently confirm these microarray findings, we assessed Dab2 gene and protein expression in C57B/6 mice during MOG-induced EAE.
To substantiate our microarray findings, we used a qRT-PCR array (SABioscience) to measure expression of TLRs, NF-κB genes, several cytokines and their receptors at 12 months of age in the Abcd1 null mice.
To validate microarray findings, we selected 3 genes (Fst, Smad3, and Myf6) that were found to be significantly affected by SCI and showed reversed changes after the initial treadmill training.
To confirm our microarray findings, we used immunohistochemistry (IHC), quantitative real-time PCR and western blot to determine the expression of NM23-H1, MMP-9 and VEGF165 in NPC tissues with and without intracranial invasion.
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To validate our microarray finding, we assayed the serum of 45 pSLE patients and 24 healthy controls by indirect serum ELISA, using recombinant BAFF as the antigen.
+ HJURP expression is measured as log2 (probe intensities) by Affymetrix microarray To validate our findings, we used several independent breast cancer cohorts with previously reported microarray data deposited in the Gene Expression Omnibus (GEO) database [ 17], to compare mRNA level of HJURP in tumor tissue with patient survival (Table 3).
In addition to confirming the expression of known markers by comparing our microarray data to previously documented findings, we verified transcript accumulation of selected factors with qRT-PCR.
Consistent with our interpretation of the microarray findings in KIKO skeletal muscle, we found a coordinate downregulation of Pgc1a and upregulation of Srebp1 (Fig. 2A,C,D).
We validated the microarray findings by quantifying irg1, ifit3, and socs3 transcripts via quantitative reverse transcription PCR (qRT-PCR) using several of the original RNA samples as templates.
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