Sentence examples for microarray findings the from inspiring English sources
To further assess biological relevance of the microarray findings, the transcription abundance of 13 genes selected for real-time PCR was determined in parasites associated with different malaria clinical backgrounds.
To validate the microarray findings, the expression levels of cathelicidin antimicrobial peptide (CAMP), alpha defensin 1 (DEFand, and carcinoembrionic antigen-related cell adhesion molecule 8 (CEACAM8) were quantified relative to the endogenous control gene, beta actin (ACTB) using pre-designed TaqMan gene expression assays (Applied Biosystems, Foster City, CA).
Consistent with our microarray findings, the activating phosphorylation of the checkpoint proteins, p-ATM, p-RAD17, and p-CHK2, was significantly higher in IR-treated CD133+ than in IR-treated CD133− (Figure 6A), indicating that CD133+ AT/RT cells display greater checkpoint activation in response to DNA damage.
To confirm the microarray findings, the results were independently validated by TaqMan gene expression assays.
As shown in figure 4 and confirming the microarray findings, the expression of dcuS, fldB, iclR and tdcA is enhanced in the ubiG mutant by ArcA.
Mirroring the microarray findings, the hormonal message in upper SI K-cells was dominated by gip, but the cells additionally contained detectable mRNA for sst, sct, cck, gcg, and pyy.
Overall, there was a good correspondence between mRNA levels estimated in the three patient subgroups by microarray and qPCR analysis and the qPCR results confirmed the microarray findings with the exception of HLA-A (r2 = 0.71, n = 21).
Expression of five of those genes was measured using RT-qPCR (Additional file 2: Figure S2), and the results for each gene were consistent with the microarray findings for the effect of 4× and day of lactation on mammary gene expression.
Over-expression and under-expression ratios were statistically consistent with the microarray findings and the overall trend of gene expression was similar for both microarray and qRT-PCR experiments for all the tested genes (Table 6), as shown by Pearson correlation (data not shown).
Real-time PCR was used to validate the microarray findings in the SK-N-Be(2 C cell lines.
These data served to both substantiate the corresponding microarray findings at the protein level, and provide further support for our interpretations of the global transcriptome data for crypt and villus epithelial cell populations.
