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HELP microarray data has been submitted to the GEO database for public access (accession number pending) Validation of HELP microarray findings was carried out by MALDI-TOF mass spectrometry using EpiTyper by MassArray (Sequenom, CA) on bisulfite-converted DNA as previously described[31].
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Our microarray findings were confirmed by quantitative realtime PCR on patient samples as well as on samples from healthy normal controls that underwent the TI procedure.
Microarray findings were validated by real-time quantitative PCR.
The microarray findings were validated using quantitative real-time RT-PCR.
Microarray findings were then corroborated and extended by real time RT-PCR in the same cohorts of subjects, as well as in an independent group of 25 MS patients and 11 healthy controls.
Hence, the microarray findings were validated.
In all cases, the microarray findings were confirmed.
Microarray findings were validated with real time RT-PCR analyses.
Key microarray findings were verified by real-time PCR and additional in vitro experiments of matrix synthesis and signal transduction.
Microarray findings were further validated by qRT-PCR analysis in another bioptic series of 75 mucosa samples.
The microarray findings were independently corroborated for a random set of 14 mRNAs by RT-PCR analysis.
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