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To confirm the results of the microarray analyses, we measured the expression of several genes using qPCR.
Using microarray analyses, we found that both pathways inhibit sexual differentiation by blocking ste11-dependent gene expression.
To verify that sRNAs are transcribed at the locations indicated by bioinformatic and microarray analyses we performed Northern blot analysis.
Surprisingly, and because of the unbiased nature of microarray analyses, we found that the most significantly changing genes were clustered in the neighbourhood of the KO gene.
Using microarray analyses, we were able to identify 818 genes out of 6221 that were expressed differently in 5A mutant compared to control cells, based on 0.5 and 2 fold expression ratios as cut-off values.
Therefore, for microarray analyses, we sub divided the LEP-ICV group into two groups: (a) LEP_ICV group: treatment worked (weight loss range: 25% 38%) and (b) LEP_ICV*: treatment did not work (weight loss −5.42% to 1.49%).
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Based on microarrays analyses, we propose that MEC constitutes an important LET-418 containing regulatory complex during C. elegans embryonic and early larval development.
We established two screening indexes for microarray analyses: first, we filtered out miRNAs with low expression in ECs and a low fold change between YECs and OECs, and second, we applied a statistical threshold of P < 0.05 (ANOVA).
For microarray analyses of growing cells, we studied CU428 cells at three different densities that, for convenience are referred to as low (L-l), medium (L-m) and high (L-h).
Via whole-genome microarray analyses of MS lesions, we observed a differential expression of numerous Nrf2-responsive genes, also involved in the defense against oxidative stress, predominantly in areas of initial myelin destruction within actively demyelinating white matter lesions.
Using the single-cell collection technique that we developed previously (Inaki et al., 2007), we performed microarray analyses of four neighboring ventral muscles, M6, M7, M12 and M13, using Affymetrix Drosophila Genome chips (see Materials and Methods for detail).
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