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Wild Type male mice were wounded and infected with 15 ul of S. aureus as described above.
For histological analysis, a total of 77 mice were wounded, from which tissue was isolated for each of the four genotypes (wild-type, Mmp13-deficient, Plau-deficient and Mmp13 Plau double-deficient).
To determine whether addition of defensin peptide can block bacterial infection similarly to that shown for sNAG treatment, wild type mice were wounded and inoculated with S. aureus as described above and then treated with biologically active human β-defensin 3 peptide (1.0 µm) for three days.
Four TAs from C57BL/10.SJL- Dysf and control C57BL/10 mice were wounded with a hypodermic syringe needle and the muscle collected after 6 h.
In parallel with the p38 phosphorylation studies described above, 15 diabetic mice were wounded as described earlier and treated with SCIO-469.
Mice were wounded 5 weeks after transplant, and wounds harvested at day 7. Consistent with previous studies (Mace et al., 2009), the number of GFP+ cells in db recipient wounds was three-to four-fold higher than non-db recipient wounds (Fig. 6A,B), independent of the donor genotype.
Similar(54)
To provide a positive control for inflammation markers, an additional mouse was wounded with a 4 mm punch biopsy as described previously [ 76], then tissue harvested using an 8 mm punch biopsy 3 days after wounding.
Thirty-two female diabetic mice with blood glucose levels around 400 mg/dl and eight female nondiabetic mice (db/+) were wounded as described above.
Male mice between 8 12 weeks were wounded as described above.
Our standard protocol calls for the removal of all mice in cages in which fighting has led to serious injuries; both dominant and nondominant, that is, wounded, mice are removed from the protocol to prevent over-representation of mice with specific personality characteristics.
Even if those ectopically developed nodules in nude mice are apically wounded in a manner mimicking the casting of antlers [ 60], no initiation of antler growth was observed).
Related(20)
mice were sustained
mice were confirmed
individuals were wounded
mice were trained
mice were administered
mice were subjected
mice were sacrificed
mice were chosen
mice were challenged
mice were housed
mice were tested
mice were used
mice were purchased
mice were treated
mice were protected
mice were generated
mice were killed
mice were monitored
mice were euthanized
mice were anesthetized
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