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Exact(7)
Freeze-dried CNPs (∼5 mg) were resuspended in 1 ml of phosphate-buffered saline solution (PBS, pH 7.4) and incubated at 37°C under light agitation of 150 rpm using an incubator shaker (Wadegati Labequip, India; n = 3).
Lyophilized bacteria (10 mg) were resuspended and sonicated as described above.
Isolated nuclei (0.5 mg) were resuspended in 100 µl (10 mM Tris pH 8.0, 5 mM MgCl2, 40% glycerol, 2.5 mM DTT).
Briefly, lyophilized cells (10 mg) were resuspended in 400 µl isobutyric acid/1M ammonium hydroxide (5∶3, v/v) and incubated in a screw-cap test tube at 100°C for 2 h, with occasional vortexing.
NPs pellets (20 mg) were resuspended in PBS to produce a NP suspension of 160 mg/mL prior to further use.
Tissue extracts (20 mg) were resuspended in 500 μl lysis buffer containing 20 mM Hepes, pH 7.4, 10 mM KCl, 1 mM dithiothreitol, 3 mM β-glycerophosphate and complete protease inhibitor cocktail (Roche, Mannheim, Germany).
Similar(53)
ECCNS (25 mg) was resuspended in 10 mL PBS and loaded in a dialysis bag.
A fraction of the collected soil sample (10 mg) was resuspended in 1 ml sterile demineralised water.
The same amount of enriched IBs (1 mg) was resuspended in 1 mL solubilization buffer (8 M urea) and vortexed for 2 h at room temperature.
100 mg was resuspended in 1 mL 0.1 M Tris/HCl pH 7.5, 1 mM EDTA, and 5 mM β-mercaptoethanol and incubated at 4°C for 15 min.
The insoluble cell wall material (about 20 mg) was resuspended in 500 μl 2 N HCl and 50 μl thioglycolic acid (Fluka).
Related(20)
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mg were coadministered
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