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Pre-weighed dried cells (5 10 mg) were boiled with 1 mL conc.
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Each sample (1 mg) was boiled for 10 min in 2 ml of 1 N sodium hydroxide.
In order to release cellular ATP, frozen tissue (25 mg) was boiled for 2 min after the addition of 300 μl of water containing 100 mM Tris/HCl (pH 7.75) and 4 mM EDTA.
Two individual plants (100 400 mg fresh weight) were boiled in 50 ml 80% (v/v) ethanol.
50 mg of dried feces were boiled in 1 ml alkaline methanol (1M NaOH/Methanol, 1∶3 v/v) at 80°C for 2 h after addition of 50 nmol 5α-Cholestane as internal standard for neutral sterol analysis.
500 mg of dried plant leaves were boiled in 10% HCl for 5 mins and filtrate was allowed to cool.
These protein solutions were boiled at 95°C for 10, 30, or 120 min. The boiled protein solutions were diluted in DMEM at final concentrations ranging from 0.02 mg/ml to 2 mg/ml for boiled BSF and from 0.04 mg/ml to 4 mg/ml for boiled HSA.
The samples were boiled for 5 minutes in 4X non-reducing sample buffer and run on a 10% polyacrylamide gel containing gelatin (4 mg/ml) [21], [22].
Alternatively, elution was performed with 5 mg/ml FLAG peptide (Sigma) and eluted proteins were boiled in sample buffer.
Embryos were boiled as described.
Samples were boiled for 12 min, cooled to RT. Proteinase K (2 μl of 20 mg/ml) was added and incubated at 55°C for 30 min while shaking.
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