Your English writing platform
Discover LudwigSuggestions(1)
Exact(2)
Roche 454-FLX libraries (400 1200 ng) were resuspended in 16 µL of hybridization buffer (50 mM Hepes pH 7.5, 500 mM NaCl) and heat denatured at 98°C for 2 minutes in a MJ Research PTC100 thermal cycler.
In brief, 8 × 104 tumor cells or 2.5 × 105 NG were resuspended in 50 μl of normal culture medium without any supplements, or in culture medium conditioned by either normoxic or hypoxic tumor cells.
Similar(58)
Approximately 25 30 ng of in vitro transcribed RNAs were resuspended in RNA re-suspension buffer (20 mM HEPES, 100 mM NaCl, 1 mM Mg OAc 2) and refolded.
For RFLP and medium indel assays, 10 ng dry PCR primers (IDT, Coralville, IA) were resuspended into 40 μM in a 96-well format.
The sFlt1 5′-GCCGCTATAAAGAATAAGGGCCTGA-3′ (5 ng) and mFlt1 5′-CAGCAGTTCACTCACATCTCCGTTC-3′ (5 ng) (Zygmunt et al., 2011) antisense morpholino oligonucleotides (Gene-tools) were resuspended and injected as described by Ben Shoham et al. (2012).
HIV-1 lysates were prepared by ultracentrifugation of each virus stock (500 ng of p24 at 20,000× g for 2 h at 4°C) and the pellets were resuspended in Laemmli buffer at a concentration of 8.3 ng of p24/µl.
Morpholinos (Gene Tools, LLC, Philomath, OR) were resuspended in 1× Danieu's solution and 5 ng injected into fertilized eggs from TuAB fish at the single-cell stage.
MOs were resuspended in diethyl pyrocarbonate (DEPC -treated DEPC -treatedck concentration of 20 ng nl−1.
qPCR primers were resuspended at 500 nM each and validated by performing a tenfold dilution curve qPCR, starting with 2000 ng cDNA, down to 2 ng cDNA, in duplicate.
Washed cells were resuspended into 1 ml of enterokinase buffer and 4 ng/ml of enterokinase (New England Biolabs, USA) was added to the cell suspension.
Media was removed and cell pellets were resuspended in 300 μl of 0.1% BSA-PBS solution containing 25 ng/ml NAO and left to incubate at 37° for 10 minutes.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com