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It was developed as a convenient method for volatile organic compounds in the early 1990s.
However, several studies have revealed instabilities with quantification by this method [Brás et al. 2011, Weldegergis et al. 2011, Zeng and Noblet 2002], so further evaluation of the quantitative use of the method for volatile mixtures from A. sarcoides was tested.
The SPE procedure is based on a recently published SPE method for volatile cleavage products of β-carotene [ 47].
Preceding offline SPE as well as the applied internal standard are shared with a GC EI MS method for volatile CPs previously published by our group [ 47].
Contrary to all other validation approaches published so far in the context of β-carotene and CPs, our groups have jointly published two comprehensive papers providing full validation of a GC-EI-MS method for volatile CPs and an UHPLC-DAD-ESI-Orbitrap MS method for non-volatile CPs and β-carotene, with preceding off-line SPE for all analytes [ 111, 116].
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To the knowledge of the authors, no validated methods for volatile CPs of BC are available, especially for their determination in cell cultures.
Fourteen polycyclic aromatic hydrocarbons (PAHs) with different volatility were used as model compounds to investigate the method performance for volatile and semi-volatile compounds.
In order to study volatile metabolic compounds, we used gas chromatography/mass spectrometry (GC/MS), a powerful method for studying volatile organic compounds.
For this purpose, the HS-SPME sampling method for the volatile compounds of A. camphorata in submerged culture was optimised by a D-optimal design.
Commonly used methods for isolating volatile components from natural sources include thermal desorption or vapor collection by cryogenic concentration or by adsorption on solid adsorbents, direct solvent extraction (e.g., Soxhlet and liquid liquid extraction) [10, 11].
Tholl et al. (2006) recently reviewed currently available methods for plant volatile collection and analysis.
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