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Approximately 50 μg of desalted crude extract was incubated in a final volume of 200 μl containing 1 mM guaiacol and 1 mM SAM in buffer A for 30 minutes at room temperature and SPME device was employed for volatile collection.
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However, it has rarely been employed for the analysis of volatile oils in natural extracts.
The unique signaling molecule employed for quorum sensing is the volatile 3-hydroxy pacidtic acid methyl ester (3-OH PAME) [60].
A combined gas chromatography mass spectrometry (GC MS) with modified headspace solid-phase microextraction (SPME) method, with the headspace inside a designed sampling apparatus instead of in a vial, has been employed for the analysis of the flavor volatiles of glutinous rice during cooking.
A calibration chamber has been designed and employed for the simple and easy determination of uptake sampling rate (RS) of volatile organic compounds (VOCs) from air using passive samplers.
An H-register, employed for saving a specific H-matrix selected from the four H-matrices, prevents accessing of the non-volatile memory frequently during the communication time.
Nor is it employed for egotistic ends.
Other contractors were employed for specialist tasks.
Two techniques were employed for mixing.
Negative controls were employed for each assay.
statistical software were employed for these analyses.
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