It was developed as a convenient method for volatile organic compounds in the early 1990s.
However, several studies have revealed instabilities with quantification by this method [Brás et al. 2011, Weldegergis et al. 2011, Zeng and Noblet 2002], so further evaluation of the quantitative use of the method for volatile mixtures from A. sarcoides was tested.
The SPE procedure is based on a recently published SPE method for volatile cleavage products of β-carotene [ 47].
Preceding offline SPE as well as the applied internal standard are shared with a GC EI MS method for volatile CPs previously published by our group [ 47].
Contrary to all other validation approaches published so far in the context of β-carotene and CPs, our groups have jointly published two comprehensive papers providing full validation of a GC-EI-MS method for volatile CPs and an UHPLC-DAD-ESI-Orbitrap MS method for non-volatile CPs and β-carotene, with preceding off-line SPE for all analytes [ 111, 116].
Commonly used methods for isolating volatile components from natural sources include thermal desorption or vapor collection by cryogenic concentration or by adsorption on solid adsorbents, direct solvent extraction (e.g., Soxhlet and liquid liquid extraction) [10, 11].
Tholl et al. (2006) recently reviewed currently available methods for plant volatile collection and analysis.
Fourteen polycyclic aromatic hydrocarbons (PAHs) with different volatility were used as model compounds to investigate the method performance for volatile and semi-volatile compounds.
This work is based on previously developed methods for semi-volatile hydrocarbon exposure from fuels usage and similarly employs liquid solvent extraction, evaporative volume reduction, and subsequent specialized gas chromatography mass spectrometry analysis (GC MS).
Due to the lack of some commercially available CP standards, validated methods for these volatiles are missing, consequently excluding standardization of CP application solutions and quantification of CPs in cell cultures.
