Sentence examples for method and maintained from inspiring English sources

EBs were formed using the handing drop method and maintained in suspension.

The Zeocin-resistant colonies were isolated by the ring cloning method and maintained in the medium supplemented with Zeocin.

Any colonies exhibiting phenotypes suggestive of mycobacteria were stained using the Ziehl-Neelsen method and maintained on the same media at room temperature and hidden from light until PRA was performed.

The Zeocin-resistant colonies were isolated by the ring cloning method and maintained in medium supplemented with 250  μg ml−1 Zeocin (Invitrogen Corp .. Medium was replaced with complete medium without antibiotic supplement at least 5 days before any analysis.

The mutant mice were generated by using a homologous recombination method and maintained on a mixed 129Sv/C57BL/6 background and housed under specific pathogen-free conditions at a constant room temperature of 22 24 °C with a 12-h light/dark cycle.

Using the Guyan reduction method and maintaining the overall shape of the structure, a freedom set with a uniform distribution was selected.

Hence the LC-SB-SA method can yield the results with similar accuracy as LC method, and maintain its good performance when number random variables is increased to 120.

The proposed multiscale coupling approach retains the advantages of domain decomposition approaches, including the use of existing solvers for each subdomain, while it gains flexibility in the choice of the numerical discretization method and maintains the coupling errors bounded by the upscaling error.

To simplify the method and maintain a heterogeneous cancer cell population, all G418 resistant cells were trypsinized and pooled together.

When investigating the cooperation of two transgenes in a transgenic mouse, generally two characterized transgenic mouse lines are crossed, but this is a time-consuming method and maintaining an appropriate level of gene expression is difficult.

Organisms were isolated using standard methods and maintained on agar slants and refrigerated for further use.