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The data derived and maintained by the OSM project can be used for different purposes [4].
The control and βIRKO β-cell lines were derived and maintained as described previously [49].
Most prior established iPS cell lines were derived and maintained on mouse embryonic fibroblast (MEF) cells supplemented with exogenous leukemia inhibitory factor (LIF).
This demonstrates that rat cells derived and maintained in the 2i growth conditions are readily amenable to genetic modification, and should encourage efforts to achieve routine gene targeting in the rat.
Unexpectedly, upon subsequent passaging, the cultures uniformly maintained a characteristic murine ES-like morphology, with round and compacted cell clusters expressing Oct4-GFP (Figures 1F 1I) which contrasts sharply with the flattened two-dimensional colony morphology of EpiSCs derived and maintained under the same culture conditions.
The primary HDF was derived and maintained as described by Makpol et al. (2011) [ 31].
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The two congenic C3H lines [ 14] (C3H rd1/rd1 and C3Hwt/wt) were re-derived and maintained at Charles River.
A colony of PKD2 ws25/w183 compound heterozygous mice (referred to herein as ws25/-) kindly provided by Albert Einstein College of Medicine, and re-derived and maintained.
We thank Susanna Colaco for deriving and maintaining all MuHV-4-specific B cell hybridomas.
We gratefully acknowledge Michael Way for advice and reagents, Jeremy Skepper of the Wellcome Trust multi-imaging centre for help with time-lapse confocal imaging and electron microscopy, and Susanna Colaco for deriving and maintaining MHV-68-specific B cell hybridomas.
Various culture conditions, including the use of feeder cells, conditioned media, growth factors, and serum have been used to derive and maintain ESCs.
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