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After incubation, the plates were inspected for presumptive colonies before Gram staining, and cells resembling with Campylobacter were subcultured onto mCCDA by streaking colony method and incubated for 2 to 5 d at 42°C under microaerophilic conditions.
0.1 ml of the solution from last three dilution was transferred to sterile nutrient agar culture media plates (pH 7) via the spread plate method and incubated at 37 °C for 24 h.
The paraffin sections were dewaxed by routine method and incubated for 10 min with 3%H2O22.
Samples were cultured on Lowenstein-Jensen media after processing by modified Petroff's method and incubated according to CLSI guidelines.
Salivary samples were cultured within 2 hours of collection on Sabouraud glucose agar plates using the streaking method, and incubated at 35°C for 24 48 hours.
After 6 days in vitro (DIV), neurons were transfected with wild-type or mutated (H246N) or (Y251S) myc-tagged CADM1 using the calcium phosphate method and incubated for 2 DIV.
Similar(53)
All strains were plated out onto appropriate medium (for details see "Materials and methods") and incubated.
Cells were incubated micro-aerobically (see "Methods") and incubated at 30 °C.
Islets were cultured as described in Materials and methods, and incubated in the presence of low (1.67 mmol/l) or high (16.7 mmol/l) glucose.
The p21, PUMA and Bax biotin-tagged promoter sequences were generated (described in our Materials and Methods) and incubated with our lysed matched clinical samples, allowing the p53 protein to bind to these sequences.
Mature P. falciparum infected erythrocytes were purified by the gelatin flotation method [30] and incubated with 1×106/ml PBMCs for the indicated time points and ratios at 37°C, 5%CO2.
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