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Organisms were isolated using standard methods and maintained on agar slants and refrigerated for further use.
Hydrous Mn oxide (poorly crystalline δ-MnO2), a Mn(IV) oxide similar to biogenic Mn oxides [57], and goethite were synthesized by standard methods and maintained as concentrated suspensions [58 60].
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We show in simulation studies that CARAT consistently outperforms existing methods and maintains high power in a wide range of population structure settings and trait models.
The simulation results confirm that the proposed scheme is able to reduce VoLTE end-to-end delay and achieve a better system capacity than current methods, and maintain the desired VoLTE QoS.
Robust surveillance, incorporating state-of-the-art microbiological, epidemiological, and biostatistical methods, and maintaining a prompt and comprehensive response to outbreaks is just as important now as it ever was.
EBs were formed using the handing drop method and maintained in suspension.
The Zeocin-resistant colonies were isolated by the ring cloning method and maintained in the medium supplemented with Zeocin.
Any colonies exhibiting phenotypes suggestive of mycobacteria were stained using the Ziehl-Neelsen method and maintained on the same media at room temperature and hidden from light until PRA was performed.
The mutant mice were generated by using a homologous recombination method and maintained on a mixed 129Sv/C57BL/6 background and housed under specific pathogen-free conditions at a constant room temperature of 22 24 °C with a 12-h light/dark cycle.
The Zeocin-resistant colonies were isolated by the ring cloning method and maintained in medium supplemented with 250 μg ml−1 Zeocin (Invitrogen Corp .. Medium was replaced with complete medium without antibiotic supplement at least 5 days before any analysis.
Using the Guyan reduction method and maintaining the overall shape of the structure, a freedom set with a uniform distribution was selected.
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