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Exact(6)
Densitometric analysis showed that PKC protein level was significantly higher in the plasma membrane fraction compared to the homogenate counterpart (Figure 1A).
The TCII-OLEO transfected cells had a dramatically higher binding with labeled B12 in the membrane fraction, compared with other transfected cells, when 57Co-labeled Cobalamin was incorporated into culture medium for three days (Fig. 1C).
The cytoplasmic mature forms of the mutant proteins were less abundant in the membrane fraction compared with the WT mature IL-1β.
Conversely, immunoblotting with antibodies against actin, a cytoskeleton protein, demonstrated approximately 20-fold depletion in the membrane fraction compared to whole platelet lysate (averaged from three independent experiments).
Forskolin challenge in GSK-3β inhibited cells caused significant increase in the TIMAP level in the membrane fraction compared to the extremely faint signal found in the same fraction of cells treated only with the kinase inhibitor.
In lungs isolated from healthy rats, acute administration of ET-1 decreased the cytosolic fraction of PKCα by 60% (p < 0.05; Figure 6a) and correspondingly tended to increase (not significant) the cell membrane fraction compared to controls.
Similar(54)
Western blots of two-phase separated membranes showed that Ntapi AHA1 was enriched with plasma membrane fractions compared to nonplasma membrane fractions, as expected.
Of interest, CD19lowIgD+ B cells exhibit higher amounts of BCR, phosphorylated CD19 and phosphorylated lyn in cytoskeletal-associated membrane fractions compared with CD19highIgD+ B cells.
Proteins that were ≥three-fold more or less abundant under biofilm (either cytoplasmic or membrane fraction) conditions compared to either mid-log or stationary conditions, were defined as biofilm-upregulated and biofilm-downregulated proteins, respectively.
Moreover, when the reactivity of the CSF against myelin and nonmyelin membrane fractions was compared, a higher reactivity against myelin was found for the MS patients, but not for OND.
The synaptic membrane markers PSD-95 and N-cadherin was enriched in the synaptic membrane fraction and of the synaptic vesicle marker synaptophysin was enriched in the synaptic vesicle fraction compared to the homogenate (Figure 2A).
Related(15)
membrane fraction measured
surface fraction compared
membrane surface compared
membrane performance compared
membrane potential compared
membrane packing compared
membrane perforation compared
membrane sink compared
membrane stability compared
membrane area compared
membrane location compared
membrane system compared
membrane preparation compared
membrane permeability compared
membrane staining compared
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