Exact(4)
In contrast the same analysis on F508del homozygous cells revealed a strong reduction of plasma membrane staining compared to that observed in the cytoplasm.
These results were confirmed by immunostaining showing that cells transfected with COMMD1 exhibited an intense plasma membrane staining compared to cells in the same field expressing COMMD1 endogenously (Figure 2D).
However, the jejunal and ileal sections of the postconditioned group presented significantly more intestinal epithelial cells with ZO-1 membrane staining compared with the IR-group (Table 3 and Figure 3).
This was also observed in 78% of tumours, although less frequently in poorly differentiated tumours, in which only 60% (12 out of 20) of cases scored positively for apical membrane staining, compared with moderately and well-differentiated tumours where 92.5% (25 out of 27) and 88% (8 out of 9) of cases, respectively, were positive for this type of staining (χ P-value=0.01).
Similar(56)
Interestingly this chimeric fusion protein did not reflect the same pattern as the aPKCζΔcatalytic with regard to membrane staining (compare Fig. 5B and Fig. 5C), thus an interplay between the remaining ζ-N-terminus and its hinge region must exist which has the capacity to drive the aPKCζ construct to the membrane.
Wnt pathway activation has been previously inferred from the altered cellular distribution of β-catenin in pancreatic adenocarcinomas, with decreased plasma membrane and increased cytoplasmic staining compared to normal pancreatic ducts and acinar cells by immunohistochemistry [46].
The staining pattern of grades 2 and 3 tumours was similar to that of grade 1 tumours, with the occurrence of more membrane staining of the epithelial cells compared to the diffuse cytoplasmic staining.
Kriegl et al. reported a significant membrane staining of TRAIL-R1 in HCC compared to normal liver tissue and a longer survival of HCC patients undergoing partial hepatectomy with TRAIL-R1 membrane positive versus negative tumors [ 31].
In univariate analysis, EAC with positive membrane staining of DR5 presented better DFS when compared to EAC negative for DR5 (p = 0.033; Fig. 5b).
Plasma membrane staining of β1-integrin was more intense in CEDNIK cells compared to control cells exhibiting a fiber network staining (Figure 6A and F).
Compared with CONs, epithelial cells with cell membrane staining of TACSTD2 were gradually decreased from CIN1 to SCC.
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