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E1AmtPIDD cells also showed a significantly reduced decrease in mitochondrial membrane potential compared to E1A+ cells.
E1AiPIDD cells when treated with NO showed only ~5% loss of mitochondrial membrane potential compared to the 50% loss observed with E1A+ cells (Fig. 4b).
Interestingly, L-type calcium channels can be activated by rather weak depolarization events (−55mV to positive)18 and cancer cells have been reported to have depolarized membrane potential compared with their healthy counterparts46.
Under Al treatment, the mitochondria showed a time-dependent decrease in inner membrane potential compared with the control (Figure 10).
Also, adenovirus-LETM1 reduced mitochondrial membrane potential compared with GFP and Control group (Fig. S1A).
In the present study, co-treatment of primary astrocytes with both cocaine and gp120 resulted in a significant decrease in mitochondrial membrane potential compared with cells treated with either agent alone.
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Overexpressed recombinant GFP-GluA1 forms homomeric receptors, which, in contrast to most endogenous AMPARs, are inwardly rectifying; that is, they display little outward current at positive membrane potentials compared with endogenous receptors.
Similarly to other mutants with comparable level of truncation, such as L31X and S33X, BC033036 had reduced activation at positive membrane potentials compared to currents elicited by the expression of wild type protein (Fig. 5B).
Therefore, direct comparison of the steady-state activation and inactivation of IKv measured under identical experimental conditions in both cell types clearly demonstrates that PASMCs have significantly greater current amplitude and more available and less inactivated Kv channels in the negative range of membrane potentials compared with MASMCs.
Consistent with a greater capacity for oxidative phosphorylation (OXPHOS), tetramethylrhodamine, ethyl ester (TMRE) staining determined that c-Kit+ neutrophils possess greater mitochondrial membrane potential when compared to c-Kit− neutrophils (Fig. 1g).
NADH became more reduced, reactive oxygen species increased and mitochondrial membrane potential fell compared to either sham serum or PSS.
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