Sentence examples for medium without horse from inspiring English sources

Oligofectamine and siRNA were separately diluted in M199/DMEM medium without horse serum, mixed and incubated for 30 min at RT. Sixteen hours after the onset of transfection, non-transfected as well as transfected cardiomyocytes were treated with 200 µM oleic acid for additional 4 h.

After 2 days, the dish volume was replaced by medium without horse serum to slow down glial proliferation.

From day 13 on EHTs were kept in serum-free medium, i.e. the above medium without horse serum plus triiodothyronine (T3, 0.5 ng/mL, European Commission Joint Research Centre IRMM-469).

From day 13 onwards EHTs were kept in serum-free medium, i.e. the above medium without horse serum plus T3 (0.5 ng/mL, European Commission-Joint Research Centre IRMM-469) and low concentrations of hydrocortisone (50 ng/mL, Sigma-Aldrich).

For IGF1 stimulation experiments, motoneurons were cultured for 5 days with 5 ng/ml BDNF in full medium, and then starved in the same medium without horse serum and BDNF for 12 h.

The overall aim of the present investigation was to assess viability, morphological and proliferation differences of primary bronchial fibroblasts of the horse subjected to basal medium without serum, or first serum containing but later serum-depleted medium, in the presence of fetal bovine serum or horse serum.

These isolates were grown in modified Friis medium containing horse serum [25].

F12 medium, DMEM, horse serum, penicillin, and streptomycin were purchased from Invitrogen (Carlsbad, CA, USA).

The shoot and root tissues were collected from control (Hoagland medium without drought stress) and 48 h stressed (induced drought stress with 18% PEG-6000 in Hoagland medium) samples of horse gram plants M-191 (drought-sensitive genotype) and M-249 (drought-tolerant genotype).

Controls were incubated in medium without lectin.

Infection was performed in medium without FCS.