Sentence examples for medium without uracil from inspiring English sources

The cells were subsequently plated on M9 minimal medium without uracil.

Therefore, only cells that took up the chromosomal DNA from B. licheniformis DSM13 ∆spoIV and complemented the ∆pyrE locus are able to grow on M9 minimal medium without uracil.

Similarly, transcript levels of all three genes rapidly dropped after transfer to medium without uracil.

Three independent transformants were grown to late log phase in SD medium without uracil and histidine.

Screening for suppressor mutants was then carried out in nitrogen-rich medium without uracil.

NAT allows easier selection on minimal medium (without uracil), which can be advantageous in situations where constructs with the active urg1 promoter lead to sick or dead cells.

The resulting transformants were verified for stable integration by growing them on normal-dose 5-FOA medium (1 g/l of 5-FOA) and minimum medium plates without uracil.

Minimal medium contained 100 ml 67 g/l sterile yeast nitrogen base, 100 ml 19.2 g/l sterile yeast synthetic dropout medium supplement without uracil, 100 ml sterile 20% raffinose, 700 ml sterile double-distilled H2O (ddH2O), and 1 ml 25 g/l chloramphenicol.

Synthetic complete (SC) dropout plates contained 100 ml 67 g/l sterile yeast nitrogen base, 100 ml 19.2 g/l sterile yeast synthetic dropout medium supplement without uracil, 20 g bacto agar, 100 ml 20% sterile glucose, 1 ml 25 g/liter chloramphenicol, and ddH2O to 1,000 ml.

Yeast cells were grown in MM4 medium (pH 4.0) without uracil, supplemented with 60 mM acetic acid.

Cells harbouring pRS416 or derived plasmids were grown in the same medium conditions and without uracil (MM4-U) to ensure plasmid segregational stability.