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Rooting occurred with regenerated shoots cultured on MS medium without plant growth regulators.
The adventitious buds rooted and regenerated into plantlets when cultured on MS medium without plant regulators.
The best root proliferation was observed on MS medium without plant growth regulator (PGR).
The adventitious buds rooted and regenerated into plantlets when cultured on MS medium without plant regulators after 1 week.
Figure 2 The adventitious buds rooted and regenerated into plantlets when cultured on MS medium without plant regulators.
Kinetin and medium without plant growth regulators though supported elongation of shoots, but responses were lower compared to BA which induced a higher number of multiple shoots at all three concentrations (0.1, 0.5 and 1.0 mg/L) (Table 1).
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Then all the explants were implanted on Murashige and Skoog (MS) [20] basal medium without any plant growth regulator (PGR) for the establishment of an initial sterile culture.
The shoot buds were transferred to MS medium without any plant growth regulators for stem elongation and rooting.
Alternatively, cells were centrifuged and washed three times in PBS (150 mM NaCl; 20 mM phosphate buffer, pH 7.2) and resuspended in fresh culture medium without the plant extract, to evaluate the leishmanicidal or leishmanistatic effect.
Xylogenic cells are cultivated either on solid or in liquid medium with or without plant growth regulators.
Mycelium of P. portentosus isolated from a basidiome in Chiang Rai Province in 2003 grew well on modified Gamborg, modified Melin Norkans, and Murashige and Skoog media at 30°C and at pH 4. In vitro fructification of P. portentosus on sorghum grain medium without a host plant is presented for the first time.
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medium without serum
medium without starvation
medium without lectin
medium without sugar
medium without cell
medium without infection
medium without uracil
medium without growth
medium without scaffold
medium without puffball
medium without yeast
medium without insulin
medium without carbon
medium without sodium
medium without supplementation
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