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Controls were incubated in medium without lectin.
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Negative (without lectin or primary antibody) and positive (known rat or human tissue sections) controls were also tested.
The adhesion rate of H. minnesotensis on lectin-treated nematodes significantly decreased compared with the control without lectin treatment (P < 0.05), which supports the hypothesis that lectins might involve in the process of recognition and adhesion to the host (supplementary fig. S6, Supplementary Material online).
Infection was performed in medium without FCS.
During mycobacterial infection, culture medium without antibiotics was used.
Cultures containing medium without antigens were negative controls.
Cells were precultured in minimal medium without sugar.
Astrocytes were analyzed in standard culture medium without starvation.
The control medium without infection was collected for use.
After 6-hour incubation at 37°C, transfection medium was replaced with RPMI 1640 medium without antibiotics.
Belitsky minimal medium without citrate (BOC medium) was prepared as described [53].
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