Sentence examples for medium lacking arginine from inspiring English sources

Uracil prototrophs were plated to 5-FOA media to select for plasmid loss and the resulting 5-FOAr colonies assessed for canavanine sensitivity by replica plating to synthetic medium lacking arginine and containing canavanine at a concentration of 60 µg/ml.

Wild-type strain (FY3027 h+) was plated either onto medium lacking arginine (-Arg) and then onto medium lacking uracil (-Ura) or directly on to double selection medium lacking both arginine and uracil (-Arg, -Ura) and mutagenised by UV (3 5 mJ, 50 80% killing).

Around 106 transformants were screened for growth on a medium lacking arginine.

To select canavanine-resistant transformants, we used solid omission medium lacking arginine (SD −Arg) containing 120 μg/ml of canavanine.

In all, 10 cells from each eight individual cultures were plated on complete medium lacking arginine to select for recombinants.

Canavanine-resistant (Can-R) mutants were selected on synthetic complete glucose (SC) medium lacking arginine and supplemented with 60 μg/ml canavanine.

Although lacking arginine, the EAAm promotes arginine synthesis (Rondanelli et al. 2011).

Stable integrants were selected on plates lacking arginine and containing 60 µg/ml canavanine.

The yeast arg11 mutant, which is deficient in Ort1p/Arg11p grows poorly on media lacking arginine.

For SILAC, DMEM lacking arginine and lysine was used, along with 10% dialyzed fetal bovine serum.

Humans fed a diet completely lacking arginine for 5 days showed no clinical signs and no toxic ammonium levels, demonstrating that we can synthesize arginine when needed [ 53].