Sentence examples for medium lacking leucine from inspiring English sources

Leu+ transformants were selected on SC medium (synthetic complete medium) lacking leucine (SC-Leu).

Yeast cells with co-transformed pDBLeu- and pPC86-derived vectors were plated and incubated on synthetic medium lacking leucine, tryptophan (DOB-Leu-Trp) and the selective medium DOB-Leu-Trp-His (supplemented with 50 mM of 3-amino-1,2,4-triazole 3-amino-1,2,4-triazole 3-amino-1,2,4-triazole 3-amino-1,2,4-triazolee His reporter gene attivity.

Transformants were plated on minimal medium lacking leucine and containing phloxin (0.02% v/v).

Yeast was grown at 30°C in either standard YPD medium or minimal medium lacking leucine (leu) to select for appropriate plasmids.

Both negative controls formed diploids that grew on SD medium lacking leucine and tryptophan but not on QDO medium (Fig. S1 (ii)).

To select double transfectants, containing a pBD and a pAD vector, the transfected yeast cells were plated on synthetic defined (SD) medium lacking leucine and tryptophan (-LW).

These colonies are then replica plated to YES medium, EMM lacking leucine (to identify Leu− colonies that have lost plasmid), and medium containing Now-U-Dead (to determine whether the Leu− colonies have regained compound sensitivity).

These transformants form colonies on the defined medium EMM lacking leucine as a result of expression of the LEU2+ selectable marker.

After transformation into the yeast strain AH109, interactions were detected by growth on several types of media: non-selective (SD-Leucine-Tryptophan, SD-LW) medium; selective medium lacking histidine, leucine, tryptophan supplemented with 5 mM of 3-aminotriazole (3-AT) (SD-LWH+3-AT); or selective media lacking histidine, leucine, tryptophan, adenine hemisulfate (SD-LWHA).

A 50 μl volume of the suspension, as well as 10×, 100×and 1000×dilutions were dropped on solid SC medium lacking either leucine, uracil and tryptophan (growth control) or histidine, leucine, uracil and tryptophan (reporter gene assay).

The double transformants were plated on selective medium lacking tryptophan, leucine and histidine and grown at 30°C for 5 days.