Sentence examples for medium lacking histidine from inspiring English sources
P. pastoris transformants were selected by the ability to grow on minimal agar medium lacking histidine due to the presence of the histidinol dehydrogenase gene (HIS4) in the transforming vector.
The yeast strains were cultivated aerobically in YPD medium (1% [w/v] yeast extract, 2% [w/v] peptone, and 2% [w/v] glucose), or in synthetic complete (SC) medium lacking histidine, adenine, leucine, and tryptophan (SC-HALW), containing 0.67% (w/v) yeast nitrogen base without amino acids, 2% glucose, Multiple Drop-outs (DSCK292; ForMedium, Norfolk, UK), and appropriate amino acids.
Yeast were transformed using lithium acetate, and transformants were isolated after growth on solid selective medium (lacking histidine).
Transformed yeast colonies grew on auxotropic medium lacking histidine and adenine (Figure 3D) suggesting that CaZF can function as a transcriptional activator.
Double transformants were patched on the same medium and replica plated on selective medium lacking histidine for auxotrophy analysis as described.
After four hours of expression, the cells were plated to medium lacking histidine to select for recombinants, and dilutions plated to YPD to determine viability.
This reporter strain was selected and maintained using minimal medium plates lacking histidine.
The yeast strain Y187 carrying pONE1-2CPA was transformed with 50 Ng of an Arabidopsis thaliana suspension culture cDNA library generated in pAct2 [ 22], selected on SD medium [ 36] lacking histidine, tryptophan and leucine and supplemented with 1 mM 3-aminotriazole (3AT).
Putative positive bait-prey interactions in the resulting diploids formed were identified by growth on quadruple dropout (QDO; SD medium lacking adenine, histidine, leucine and tryptophan) medium.
The transformed yeast cells were selected on standard defined medium lacking adenine, histidine, leucine, and tryptophan.
The yeast strain (pJ69-4A), selection medium SC-AHLW (synthetic complete medium lacking adenine, histidine, leucine, and tryptophan), culture conditions, and transformation protocol used are described in James et al. (James et al., 1996).
