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Our finding that conditioned medium from differentiated EC is not capable of triggering the same response as EPC-CM underlines the unique composition of the latter.
A total of 30 μl of medium from differentiated U937 cells treated with PMA/LPS/TFM-C or LPS/PMA were analyzed.
Cells were plated on poly- l-lysine-coated glass coverslips and exposed to unconditioned DMEM-F-12, 10% FBS medium, or conditioned medium from differentiated adipocytes.
NRG4 was detected by ELISA in conditioned medium from differentiated brown adipocytes and was undetectable in unconditioned medium and medium from preadipocytes (Fig. 8 D ).
Very interestingly, a significant increase in proliferation of Sox-2+ neural progenitors was observed in cultures exposed to the hESC-produced proteins, an effect that was lost when NPCs were cultured in control medium from differentiated cells.
Similar(55)
At the same time, conditioned medium obtained from differentiated endothelial cells (HUVEC-CM) did not change the expression levels of PDGFRβ.
Notably, conditioned medium obtained from differentiated EC, and rich in PDGF-BB, was not able to induce expression of PDGFRβ in EC.
Albumin production in the medium from the differentiated cells and primary mouse hepatocytes was determined by mouse Albumin ELISA Quantitation Kit (Alpha Diagnostic Intl. Inc, San Antonio, TX) according to the manufacturer's instructions.
Nuclear extracts from ESCs grown in heavy medium were compared to extracts from differentiated ESCs grown in light medium.
Culture medium from SSCs and the differentiated cells was collected over 2 days from equivalent numbers of cells.
In a set of experiments, Lee and colleagues have shown that rat primary astrocytes take up a-Syn aggregates (1) from conditioned medium containing a-Syn aggregates collected from differentiated SH-SY5Y cells and (2) when co-cultured with SH-SY5Y cells that overexpress a-Syn.
Related(17)
medium from various
media from differentiated
medium from tailbud
medium from p2ΦC31-transfected
medium from apoptotic
medium from live
medium from PI16-overexpressing
medium from gastric
medium from traditional
medium from acrylic
medium from unstimulated
medium from constrained
medium from uninfected
medium from transfected
medium from human
medium from confluent
medium from hypoxic
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