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C. glutamicum strains were cultured in Brain–Heart Infusion (BHI) broth (Beckton Dickinson, NJ, USA) at 30 °C, and 25 μg/mL of Km was added to the medium as appropriate.

Fresh working solutions were prepared at the time of treatment by diluting the stock solution with growth, induction or basal maintenance medium as appropriate.

Live cell imaging and FRAP analysis were performed in the continued presence of latrunculin B or K+-depleting medium, as appropriate.

Human tumor cell lines (ATCC, Manassas, VA) were grown in RPMI 1640 and Dulbecco's modified Eagle medium as appropriate (DMEM, Cellgro, Mediatech Herndon, VA) supplemented with 10% fetal bovine serum (FBS, Mediatech) and 1% Antibiotic-Antimycotic (GIBCO BRL, Invitrogen, Carlsbad, CA) according to ATCC recommendations.

The pH was adjusted to 7.4 with 0.1 M HCl and the stock 1 m M solution diluted with serum-free medium as appropriate.

Twenty-four hours before exposure to androgen, medium was replaced with 'starvation medium' consisting of phenol red-free RPMI or DMEM medium as appropriate supplemented with 5% charcoal-stripped foetal bovine serum (First Link UK).

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Similar(52)

To assay for growth in phenotypic spotting assays, five-fold serial dilutions of saturated overnight yeast cultures grown in YPD medium, or in synthetic complete medium supplemented as appropriate for plasmid selection, were plated onto appropriate media at a starting OD600 of 0.5.

"It's another when it's presented to you in this medium as an appropriate means of communication".

The CRC lines were cultured under standard conditions with 10% (v/v) bovine serum in the medium, and split as appropriate before confluence was reached.

Each overnight culture was standardised to achieve an initial concentration of 106 CFU ml-1 and inoculated into four separate wells of a 100 well sterile honeycomb plate (ThermoLabsystems) containing fresh medium and antibiotics as appropriate.

For these experiments, a second inoculum was prepared by adding 1 mL of the test tube inoculum to a 500 mL baffled Erlenmeyer flask containing 100 mL of BG-11 medium and antibiotics as appropriate.

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