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Ampicillin (100 mg/l), tetracycline (12.5 mg/l), or chloramphenicol (40 mg/l) was added to the media as appropriate.
The next day, cultures were diluted to a final OD595 of 0.1 in either YPD, nitrogen-limited media, or carbon-limited media as appropriate.
To assay strains containing plasmids, the cultures were pre-grown in -Leu, -Trp, or -Ura minimal media, as appropriate, to maintain selection of the plasmid.
Yeast cells were cultivated in liquid or on solid agar plates of rich (YPD, 1% yeast extract, 2% tryptone or peptone, 2% dextrose) or synthetic (Drop Out Mix Minus various ingredients, United States Biological) media, as appropriate to maintain plasmid selection.
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Higher harmonics can also be generated with appropriate media, as well as combinations of frequencies when two or more light beams are used as input.
Following this significant relationship with the IPL and BCCI, Live Current will be speaking with other cricket organizations and determining relationships for content, media access and distribution as appropriate.
59 61 Our findings suggest that Aboriginal children may face barriers to accessing appropriate care for otitis media, as well as the possible overuse of procedures among advantaged children.
Culture media were supplemented with Ampicillin (100 μg ml−1) as appropriate.
The composite deposition was carried out by cyclic voltammetry, potentiostatic or galvanostatic electrolysis of suspensions of the RuO2 or IrO2 particles in two different media described as appropriate for WO3 deposition: (i) a colloidal suspension obtained from Na2WO4 and H2SO4 or (ii) a solution obtained by dissolving W powder in aqueous H2O2.
Media were supplemented with 150 µg/ml G418, 50 µg/ml CloNat, or 400 µg/ml hygromycin, as appropriate.
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