Sentence examples for vector as appropriate from inspiring English sources
Data are expressed relative to the parent vector or promoterless vector, as appropriate.
They were then transfected by electroporation at 220 V and 950 μF with the PRE2/TATA-luciferase reporter along with CMV/Renilla as an internal control, and with PR-B, PR-A, or AR expression vectors as appropriate.
MDA-MB-231, MDA-MB-453, and HeLa cells were co-transfected with pMirTarget-MST4, miR-4728-3p mimic, mimic negative control, and/or pMirTarget-Vector control as appropriate using Lipofectamine 2000.
Wild-type HeLa cells were also transfected with an AR expression vector (50 ng/dish) as appropriate.
The total amount of transfected DNA was kept constant by co-transfecting the appropriate empty vector as necessary.
PCR was next used to add the proper att sequences such that fragments could be moved into the appropriate Gateway DONR vector, as described in the Gateway Protocol.
After 48 h incubation at 25 °C, the nitrocellulose discs were transferred, as appropriate for each vector, onto selection plates containing BM agar amended with 10 μg/mL sulfonylurea or YPD agar containing 200 μg/mL geneticin (G418 sodium salt) or hygromycin B and antibiotics (100 μg/mL ampicillin, 100 μg/mL cefotaxime, 100 μg/mL streptomycin and 100 μg/mL timentin).
Control MCF10A cell lines were generated by expressing empty vectors conferring puromycin or blasticidin gene resistance as appropriate.
Appropriate restriction sites can then be added, a cloning vector selected and the entire sequence synthesized or cloned, as appropriate.
Using this criterion, we selected the correlation coefficient and Simpson similarity index as appropriate distance measures depending on the sparseness of the phyletic vectors in this work.
