Sentence examples for medium as necessary from inspiring English sources

The medium was replaced with fresh medium as necessary, and cultures were maintained at 37°C in the presence of 5% CO2.

After 2 h, non-adherent cells were washed away and the adherent fraction was cultured in RPMI with GM-CSF (100 ng/mL) and IL-4 (40 ng/mL) (Biosource), refreshing the medium as necessary.

Carbon and nitrogen are the most important factor in the BS production medium, as it is necessary for microbial growth and the synthesis of enzymes and proteins depends on it.

The pH of the medium was adjusted as necessary using 5 M HCl or KOH.

Cell culture medium was changed as necessary until colonies formed.

Yeast strains and plasmids used in this study are listed in Tables 1, 2 and 3. Yeast strains were incubated in YPD (rich medium), SC (synthetic complete medium with amino acids omitted as necessary for plasmid selection), sporulation medium or SLAD (sigma strains) [14] at 30°C unless otherwise stated.

Cook 4 thinly sliced carrots in 2 tablespoons olive oil and 2 tablespoons water over medium-high heat, stirring as necessary, until the carrots are tender and lightly browned, 8 to 10 minutes.

l-Tryptophan was prepared as a sterile stock solution and added as necessary to the medium before fermentation.

Growth was in YPD or in synthetic defined (SD) medium with 2% (w/v) galactose, supplemented as necessary [ 59].

Callus cultures were maintained on solid medium (MS medium plus 0.8% (w/v) agar) in the dark and transferred as necessary.

The Fed is committed to achieving medium-term price stability and will act as necessary to attain that objective".