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The horizontal and vertical trends were removed from the data set with the median polish algorithm.

Raw data were subjected to the frozen Robust Multi-Array Average (fRMA) algorithm56, followed by summarization based on multi-array model, fitted using the Median Polish algorithm.

Data were normalized [21] and summarized using Median Polish algorithm.

This utilized a three-step approach of background correction on PM (Perfect Match) data, quantile normalization, and Tukey's median polish algorithm for summarization of probe level data.

Robust estimates in the linear model are obtained by using the median polish algorithm.

For the single-color mRNA data, data preprocessing steps included RMA background correction [ 43], quantile normalization [ 44] and summarization of mRNA expression using the median polish algorithm.

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This accumulating sum of best one-dimensional approximations is an algorithm for finding a best approximation from U. Variations of the method have been called Von Neumann alternating search, Diliberto-Strauss, and median polish algorithms.

Finally, a median polishing algorithm was used to summarize the background-corrected and the normalized probe intensities to generate a final probe set value.

Finally, a median polishing algorithm is used to summarize the background-corrected, normalized probe intensities to generate a final probe set value.

The data output was analyzed following a per chip and per gene median polishing algorithm and quality assessment of the normalization procedure performed by Boxplot and MA plot visualization.

Quantile normalization [ 19] and Tukey's median-polish algorithm based gene summary values were computed using a modified C program capable of processing several thousand GeneChips simultaneously (L. Bao, unpublished results).

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