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Moreover, the lipid peroxidation levels and antioxidative capabilities were measured using biochemical analysis.

Activities of total-SOD, GSH-Px, and iNOS and MDA concentration were measured using biochemical analytic kits.

Of the 172 patients, 16.3% had ER measured using biochemical methods and 83.7% had ER measured using immunohistochemistry. ER, estrogen receptor; HRT, hormone replacement therapy; -, negative; +, positive.

The level of glutamate-oxaloacetate transaminase (GOT) and glutamate-pyruvate transaminase (GPT) in plasma was measured using biochemical analyzer (AU400; Olympus, Tokyo, Japan).

Activities of blood serum marker enzyme including alanine transaminase (ALT), aspartate aminotransferase (AST), triglyceride (TG), and total cholesterol were measured using biochemical analyzer (Hitachi 902 Automatic Analyzer) and adapted reagents from Roche (Germany).

Tissue synthesis was assessed by measuring sGAGs, which can be easily measured using biochemical assays, where sGAGs synthesized and secreted by chondrocytes are in the form of proteoglycans, with the majority being aggrecan.

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Plasma biochemical indicators were measured using a biochemical automatic analyzer Konelab 20i (ThermoScientific, Czech Republic) and commercial test kits (BioVendor, Czech Republic).

Different blood parameters, including white blood count (WBC), red blood cell count (RBC), hemoglobin (Hb), alanine aminotransferase (ALT), aspartate aminotransferase (AST), creatinine (Cr), and urea (BUN), were measured using a biochemical autoanalyzer (Type 7170, Hitachi, Tokyo, Japan).

The oxidative activity of tpx mutant was significantly reduced when measured using a biochemical assay with the cell extract compared to the WT strain (Fig. 6).

After 5 min of centrifugation, total amount of DNA was detected using PicoGreen Assay (Molecular Probes), while ALP activity was measured using a biochemical assay (SensoLyte pNPP ALP assay kit; ANASPEC).

Lipids (total cholesterol [TC], triglycerides [TG], low density lipoprotein cholesterol [LDL-C], and HDL-C), serum albumin, uric acid, alanine aminotransferase, magnesium, and hypersensitive-C reactive protein (hs-CRP) were measured using a biochemical autoanalyzer (Hitachi 7600 automated analyzer, Tokyo, Japan).

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