The transcriptome of a B. cenocepacia population resulting directly from the pathogen-host interaction in sputum from three CF individuals was measured using multiple biological replicates.
The basis of our comparison was a DNA microarray array experiment in which the gene expression in two different haploid strains of S. cerevisiae, RM11-1a and BY4716, in two different growth media was measured using six biological replicates (GEO accession number GSE9376) [ 11].
(588) Historically, transport and accumulation were first measured using cells (section ) and biological sheets (section ).
Diclofenac (10 or 50 µM) was added the following day and after 4 days of treatment, cells content was measured using a cell proliferation kit (Biological Industries, Israel).
The absorbance for biological assays was measured using a GBC Cintra 2010 UV VIS spectrophotometer at different wavelengths: 517, 734 and 695 nm.
Such interactions can reduce the bioaccessibility and bioavailability of peptides, and can also alter their biological activities when measured using in vitro assays.
Biological titers were measured using an Adeno-X-rapid titer kit (Clontech, Mountain View, CA).
Cell proliferation was measured using the XTT cell proliferation kit (Biological Industries) according to the manufacturer's protocol.
Three-dimensional second-harmonic fields, sample orientation, and susceptibility ratios of biological samples are measured using polarization-resolved second-harmonic generation (SHG) microscopy.
The biological responsiveness was measured using both conventional immunological assays as in the previously published literature, and whole genome expression profiling, in an attempt to understand the pathophysiology of the illness in terms of the complex biological networks.
