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Human IgG subclasses were measured using human IgG Subclass Profile ELISA kits (InVitrogen).
Concentrations of Aβ40 in QDAβ were measured using Human β Amyloid 1 40 ELISA KIT (Biosource).
Gene expression profiles measured using human genome Affymetrix Gene Chip arrays were grouped by hierarchical clustering, and correlation coefficients were computed for all pair-wise comparisons (Fig. 4A).
Enzymatic properties of cynomolgus and rhesus monkey CYP2C93, along with cynomolgus monkey CYP2C8, CYP2C43, CYP2C75, and CYP2C76, were measured using human CYP2C substrates, diclofenac, flurbiprofen, paclitaxel, S-mephenytoin, and tolbutamide.
Anticoagulant activity was measured using human or rabbit plasma.
Serum insulin was measured using human chemiluminescent immunoassay Immulite-20000, Diagnostic Products, Los Angeles, CA).
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The media were collected and secreted human wild type proinsulin was measured using human-specific proinsulin radioimmunoassay.
TNF-α, IL-10, and IL-6 were measured using human-specific Luminex™ beadsets (BioSource-Invitrogen, Austin, TX) using a Luminex™ 100 IS apparatus (MiraiBio, Austin, TX) or commercially available, pig-specific ELISA kits (R&D Systems, Minneapolis, MN) using a standard ELISA reader.
AR activity was measured using HepG2 human hepatoma cells transfected with human AR plus an androgen-responsive luciferase reporter gene, MMTV-luc.
Recombinant human Gas6 (Amgen) grown in vitamin K-containing medium was measured using the human Gas6 Quantikine ELISA kit (DGAS60, R&D Systems) following the manufacturer's protocol.
In addition, toxicities of selected ionic liquids have been measured using a human cancer cell line.
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