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Levels of thiobarbituric acid reactive substances (TBARS) were estimated using biochemical assay kit (ZeptoMetrix) by measuring the lipid peroxidation products, malondialdehyde (MDA) equivalents.
Oxidative status can be estimated using biochemical assays, such as the 2,2′-azino-bis 3-ethylbenzthiazoline-6-sulphonic 2,2′-azino-bis 3-ethylbenzthiazoline-6-sulphonic 2,2′-azino-bis 3-ethylbenzthiazoline-6-sulphonic 2,2′-azino-bis 3-ethylbenzthiazoline-6-sulphonic 2,2′-azino-bis 3-ethylbenzthiazoline-6-sulphonic 2,2′-azino-bis 3-ethylbenzthiazoline-6-sulphonic
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The percentage of surviving neurons in the presence of PDGF and /or HIV-1 Tat was estimated using the biochemical and histological analyses listed below.
The serum was separated and levels of ALT, AST, and ALP were estimated using an automated biochemical analyzer (Beckman Coulter, California, USA).
For example, with the obtained timescales and kinetic parameters, the response of special signaling activities can be estimated using ODE, beginning with listing all biochemical transformations and thereby providing a kinetic scheme of signaling pathways [32].
Biochemical recurrence-free survival was estimated using the Kaplan Meier method and was compared by using the log-rank test.
The relative fitness of each amino acid substitution is estimated using a Grantham score [ 74], which evaluates biochemical dissimilarity (based on polarity, amino acid size, and side chain composition) between ancestral and derived states, with lower Grantham scores indicating a greater biochemical similarity.
Association of demographical, clinical and biochemical variables with relapse will be estimated using univariate and multivariabel Cox proportional hazard regression analysis.
The genomic composition of DNA was estimated using quantitative polymerase chain reaction (PCR) using a Lightcycler (Roche Molecular Biochemicals) at each stage of preparation.
Biochemical relapse-free (BRFS) and local relapse-free (LRFS) survival was estimated using Kaplan Meier curves.
1/S' was estimated using BlastPhen.
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